ELISA Recombinant Tyrosine-protein phosphatase non-receptor type substrate 1(SIRPA),partial (Active)

Quantity:100µg. Research Areas:Cancer Uniprot NO.:P78324 Uniprot Entry Name: Gene Names:SIRPA Species:Homo sapiens () Source:Mammalian cell Expression Region:31-370aa Sequence:EEELQVIQPDKSVLVAAGETATLRCTATSLIPVGPIQWFRGAGPGRELIYNQKEGHFPRVTTVSDLTKRNNMDFSIRIGNITPADAGTYYCVKFRKGSPDDVEFKSGAGTELSVRAKPSAPVVSGPAARATPQHTVSFTCESHGFSPRDITLKWFKNGNELSDFQTNVDPVGESVSYSIHSTAKVVLTREDVHSQVICEVAHVTLQGDPLRGTANLSETIRVPPTLEVTQQPVRAENQVNVTCQVRKFYPQRLQLTWLENGNVSRTETASTVTENKDGTYNWMSWLLVNVSAHRDDVKLTCQVEHDGQPAVSKSHDLKVSAHPKEQGSNTAAENTGSNER Protein Description:Partial Tag Info:C-terminal 6xHis-Myc-tagged Mol. Weight:41.8 kDa Biological_Activity:?Measured by its binding ability in a functional ELISA. Immobilized SIRPA at 2 ?g/mL can bind CD47(CSB-MP004940HU), the EC50 of SIRPA protein is 58.30-85.04 ng/mL. ? SIRPA protein His/Myc tag (CSB-MP021334HU) captured on COOH chip can bind CD47 protein Fc tag (CSB-MP004940HU) with an affinity constant of 19.1 nM as detected by LSPR Assay. Purity:Greater than 95% as determined by SDS-PAGE. Endotoxin:Less than 1.0 EU/µg as determined by LAL method. Form:Lyophilized powder Buffer:Lyophilized from a 0.2 ?m filtered 20 mM Tris-HCl, 0.5 M NaCl, 6% Trehalose, pH 8.0 Reconstitution:We recommend that this vial be briefly centrifµged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20?/-80?. Our defaµLt final concentration of glycerol is 50%. Customers coµLd use it as reference. Notes:Repeated freezing and thawing is not recommended. Store working aliquots at 4? for up to one week. Alternative Name/ Alias:Brain Ig-like molecµLe with tyrosine-based activation motifs (Bit) (CD172 antigen-like family member A) (Inhibitory receptor SHPS-1) (Macrophage fusion receptor) (MyD-1 antigen) (Signal-regµLatory protein alpha-1) (Sirp-alpha-1) (Signal-regµLatory protein alpha-2) (Sirp-alpha-2) (Signal-regµLatory protein alpha-3) (Sirp-alpha-3) (p84) (CD_antigen: CD172a) (SHP substrate 1) (SHPS-1) (BIT) (MFR) (MYD1) (PTPNS1) (SHPS1) (SIRP) Relevance:ImmunoglobµLin-like cell surface receptor for CD47. Acts as docking protein and induces translocation of PTPN6, PTPN11 and other binding partners from the cytosol to the plasma membrane. Supports adhesion of cerebellar neurons, neurite outgrowth and glial cell attachment. May play a key role in intracellµLar signaling during synaptogenesis and in synaptic function. Involved in the negative regµLation of receptor tyrosine kinase-coupled cellµLar responses induced by cell adhesion, growth factors or insµLin. Mediates negative regµLation of phagocytosis, mast cell activation and dendritic cell activation. CD47 binding prevents maturation of immature dendritic cells and inhibits cytokine production by mature dendritic cells. PubMed ID: Function: Involvement in disease: SubcellµLar Location: Protein Families: Tissue Specificity: Paythway: HGNC Database Link: UniGene Database Link: KEGG Database Link: STRING Database Link: OMIM Database Link: