ELISA Recombinant Interleukin-33 protein(IL33) (Active)

Quantity:100µg. Research Areas:Immunology Uniprot NO.:O95760 Uniprot Entry Name:IL33_ Gene Names:IL33,C9orf26,IL1F11,NFHEV Species:Homo sapiens () Source:E.Coli Expression Region:112-270aa Sequence:SITGISPIT EYLASLSTYN DQSITFALED ESYEIYVEDL KKDEKKDKVLLSYYESQHPS NE SGDGVDGK mLMVTLSPTK DFWLHANNKE HSVELHKCEKPLPDQAFFVL HNMHSNCVSF ECKTDPGVFI GVKDNHLALI KVDSSENLCT ENILFKLSET Protein Description:Partial Tag Info:Tag-Free Mol. Weight:17.9 kDa Biological_Activity:FµLly biologically active when compared to standard. The ED50 as determined by a cell proliferation assay using murine D10S cells is less than 0.05 ng/mL, corresponding to a specific activity of ? 2.0 × 107 IU/mg. Purity:>97% as determined by SDS-PAGE and HPLC. Endotoxin:Less than 1.0 EU/µg as determined by LAL method. Form:Lyophilized powder Buffer:Lyophilized from a 0.2?m filtered 20 mM PB, 150 mM NaCl, 1mM EDTA, pH7.4 Reconstitution:We recommend that this vial be briefly centrifµged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20?/-80?. Our defaµLt final concentration of glycerol is 50%. Customers coµLd use it as reference. Notes:Repeated freezing and thawing is not recommended. Store working aliquots at 4? for up to one week. Alternative Name/ Alias:IL-33, IL-1F11, Nuclear factor from high endothelial venµLes, NF-HEV, Relevance:Cytokine that binds to and signals throµgh the IL1RL1/ST2 receptor which in turn activates NF-kappa-B and MAPK signaling pathways in target cells (PubMed:16286016). Involved in the maturation of Th2 cells inducing the secretion of T-helper type 2-associated cytokines. Also involved in activation of mast cells, basophils, eosinophils and natural killer cells. Acts as a chemoattractant for Th2 cells, and may function as an "alarmin", that amplifies immune responses during tissue injury (PubMed:17853410, PubMed:18836528). {ECO:0000269|PubMed:16286016, ECO:0000269|PubMed:17853410, ECO:0000269|PubMed:18836528}.; In quiescent endothelia the uncleaved form is constitutively and abundantly expressed, and acts as a chromatin-associated nuclear factor with transcriptional repressor properties, it may sequester nuclear NF-kappaB/RELA, lowering expression of its targets (PubMed:21734074). This form is rapidely lost upon angiogenic or proinflammatory activation (PubMed:18787100). {ECO:0000269|PubMed:18787100, ECO:0000269|PubMed:21734074}. PubMed ID:12819012; 10566975; 16286016; 21454686; 14702039; 15164053; 15489334; 22307629; 17853410; 17185418; 18787100; 18836528; 19596270; 19559631; 19465481; 19439663; 21734074; 22215666; 19836339; 23980170 Function:Cytokine that binds to and signals throµgh the IL1RL1/ST2 receptor which in turn activates NF-kappa-B and MAPK signaling pathways in target cells Involvement in disease: SubcellµLar Location:Nucleus, Chromosome, Cytoplasmic vesicle, secretory vesicle, Secreted Protein Families:IL-1 family Tissue Specificity:Expressed at high level in high endothelial venµLes found in tonsils, Peyer patches and mesenteric lymph nodes. Almost undetectable in placenta. Paythway:Necroptosis HGNC Database Link:https://www.genenames.org/cgi-bin/gene_symbol_report?hgnc_id=HGNC:16028 UniGene Database Link:https://www.ncbi.nlm.nih.gov/UniGene/clust.cgi?ORG=Hs&CID=731660 KEGG Database Link:https://www.genome.jp/dbget-bin/www_bget?hsa:90865 STRING Database Link:https://string-db.org/network/9606.ENSP00000370842 OMIM Database Link:https://www.omim.org/entry/608678608678608678