ELISA Phospho-NF-kB p65 (Ser281) anti-

Quantity:100µL Host:Rabbit Clonality:Polyclonal Isotype:IgG GeneName:RELA GeneID:5970 Uniprot:Q04206 Specificity:Phospho-NF-kB p65 (Ser281) Antibody detects endogenous levels of NF-kB p65 only when phosphorylated at Serine 281. Immunogen:A syntheQuantityd peptide derived from NF- kappaB p65 around the phosphorylation site of Ser281. Storage:Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.Store at -20 °C.Stable for 12 months from date of receipt. Reactivity:,Mouse,Rat Prediction:Pig,Bovine,Horse,Sheep,Dog,Xenopus Application:WB,IHC,IF/ICC,ELISA(peptide) Dilution:WB 1:1000, IHC 1:50-1:200, IF/ICC 1:100-1:500, ELISA(peptide) 1:20000-1:40000 Conjµgate:Unconjµgated RRID:AB_2834824 An Introduction to Blocking Peptides:A superior strategy for validation of antibody binding is the use of blocking peptides. In a Western Blotting assay, our blocking peptide was validated to block the signal of this antibody. This ensures the site-specificity of an antibody, including phosphor-antibodies. In the case of the phosphor- antibodies, the antibodies can and will only recognize the phosphorylated target protein at the corresponding site, not the non-phosphorylated target protein or target protein phosphorylated at a different site. Cat# Blocking Peptide:AF3393-BP Des# Blocking Peptide:Phospho-NF-kB p65 (Ser281) Blocking peptide Blocking Peptide Price:$549 CAD