ELISA Protein dispatched homolog 2 (DISP2)

Reactivity: (Homo sapiens) UniProt:A7MBM2 Abbreviation:DISP2 Alternative Names:DISPB; DKFZp547N223; HsT16908; KIAA1742; dispatched B Application:ELISA Range:Request Information Sensitivity:Request Information Intra-AssayCV:?5.7% Inter-AssayCV:?9.1% Recovery:0.99 Sample Type:Serum, Plasma, Other biological fluids Detection Method:Sandwich Analysis Method??:Quantitive Test principle:This assay employs a two-site sandwich ELISA to quantitate DISP2 in samples. An antibody specific for DISP2 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyDISP2 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjµgated antibody specific for DISP2 is added to the wells. After washing, Streptavidin conjµgated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of DISP2 bound in the initial step. The color development is stopped and the intensity of the color is measured. Product Overview:The pattern of cellµLar proliferation and differentiation that leads to normal development of embryonic structures often depends upon the localized production of secreted protein signals. Cells surrounding the source of a particµLar signal respond in a graded manner according to the effective concentration of the signal, and this response produces the pattern of cell types constituting the mature structure. A segment-polarity gene known as dispatched has been identified in Drosophila and its protein product is required for normal Hedgehog (Hh) signaling. This gene is one of two homologs of Drosophila dispatched.Ma et al. (2002) isolated cDNAs encoding 2 murine homologs of dipatched, which they called Dispa and Dispb. They predicted that the dispatched proteins all have 12 transmembrane spans with cytoplasmic N and C termini. Stability:The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. The loss rate was determined by accelerated thermal degradation test. Keep the kit at 37°C for 4 and 7 days, and compare O.D.values of the kit kept at 37°C with that of at recommended temperature. (referring from China Biological Products Standard, which was calcµLated by the Arrhenius equation. For ELISA kit, 4 days storage at 37°C can be considered as 6 months at 2 - 8°C, which means 7 days at 37°C equaling 12 months at 2 - 8°C).