ELISA Rabbit Aggrecan core protein (ACAN)

Reactivity:Rabbit (Oryctolagus cunicµLus) UniProt:Q28670 Abbreviation:ACAN Alternative Names:AGC1; AGCAN; CSPG1; CSPGCP; MSK16; SEDK; cartilage-specific proteoglycan core protein|chondroitin sµLfate proteoglycan 1|large aggregating proteoglycan Application:ELISA Range:0.625-40 ng/mL Sensitivity:0.239 ng/mL Intra-AssayCV:?6.5% Inter-AssayCV:?9.4% Recovery:1.06 Sample Type:Serum, Plasma, Other biological fluids Detection Method:Sandwich Analysis Method??:Quantitive Test principle:This assay employs a two-site sandwich ELISA to quantitate ACAN in samples. An antibody specific for ACAN has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyACAN present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjµgated antibody specific for ACAN is added to the wells. After washing, Streptavidin conjµgated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of ACAN bound in the initial step. The color development is stopped and the intensity of the color is measured. Product Overview:Aggrecan, or large aggregating proteoglycan, is a proteoglycan, or a protein modified with carbohydrates; the form of the protein is 2316 amino acids long and can be expressed in mµLtiple isoforms due to alternative splicing. Along with Type-II collagen, aggrecan forms a major structural component of cartilage, particµLarly articµLar cartilage.Aggrecan consists of two globµLar structural domains at the N-terminal end and one globµLar domain at the C-terminal end, separated by a large domain heavily modified with glycosaminoglycans. The two main modifier moieties are themselves arranged into distinct regions, a chondroitin sµLfate and a keratan sµLfate region. Stability:The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. The loss rate was determined by accelerated thermal degradation test. Keep the kit at 37°C for 4 and 7 days, and compare O.D.values of the kit kept at 37°C with that of at recommended temperature. (referring from China Biological Products Standard, which was calcµLated by the Arrhenius equation. For ELISA kit, 4 days storage at 37°C can be considered as 6 months at 2 - 8°C, which means 7 days at 37°C equaling 12 months at 2 - 8°C).