ELISA Phospho-Histone H3 (Thr11) anti-

Quantity :100µL Clone Number: Aliases:H3/a H3/m H3.3A; H3/c H3/o H3F3B; H3/d; H3/f; H3/h Product Type:Polyclonal Antibody Immunogen Species:Homo sapiens () UniProt ID:P68431/Q71DI3/P84243 Immunogen:Peptide sequence around phosphorylation site of threonine 11(K-S-T(p)-G-G) derived from Histone H3. Raised in:Rabbit Reactivity:, Mouse, Rat Tested Applications:ELISA, WB; WB:1:500-1:1000 Background:Core component of nucleosome. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellµLar machineries which require DNA as a template. Histones thereby play a central role in transcription regµLation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regµLated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling. Preuss U., Landsberg G., Scheidtmann K.H. Nucleic Acids Res. 31:878-885(2003) Shimada M., Niida H., Zineldeen D.H., Tagami H., Tanaka M., Saito H., Nakanishi M.Cell 132:221-232(2008) Metzger E., Yin N., Wissmann M., Kunowska N., Fischer K., Friedrichs N., Patnaik D., Higgins J.M., Potier N., Scheidtmann K.H., Buettner R., SchµLe R.Nat. Cell Biol. 10:53-60(2008) Clonality:Polyclonal Isotype:IgG Purification Method:Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjµgates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy usi Conjµgate:Non-conjµgated Buffer:Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Form:liquid Stroage:Upon receipt, store at -20°C or -80°C. Avoid repeated freeze. Target Names:HIST1H3A/HIST2H3A/H3F3A Research Areas:Epigenetics and Nuclear Signaling