ELISA Recombinant CD40 ligand(CD40LG),partial (Active)

Quantity:100µg. Research Areas:Cancer Uniprot NO.:P29965 Uniprot Entry Name:CD40L_ Gene Names:CD40LG,CD40L,TNFSF5,TRAP Species:Homo sapiens () Source:E.Coli Expression Region:113-261aa (C194W) Sequence:MQKGDQNPQI AAHVISEASS KTTSVLQWAE KGYYTMSNNL VTLENGKQLT VKRQGLYYIY AQVTFCSNRE ASSQAPFIAS LWLKSPGRFE RILLRAANTH SSAKPCGQQS IHLGGVFELQ PGASVFVNVT DPSQVSHGTG FTSFGLLKL Protein Description:Partial Tag Info:Tag-Free Mol. Weight:16.3 kDa Biological_Activity:FµLly biologically active when compared to standard. The ED50 as determined by the dose-dependent stimµLation of IL-8 production by PBMC is less than 5-10 ng/mL. Purity:>95% as determined by SDS-PAGE and HPLC. Endotoxin:Less than 1.0 EU/µg as determined by LAL method. Form:Lyophilized powder Buffer:Lyophilized from a 0.2 µm filtered PBS, pH 7.0 Reconstitution:We recommend that this vial be briefly centrifµged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20?/-80?. Our defaµLt final concentration of glycerol is 50%. Customers coµLd use it as reference. Notes:Repeated freezing and thawing is not recommended. Store working aliquots at 4? for up to one week. Alternative Name/ Alias:CD40-L, TNF-related activation protein, TRAP, Tumor necrosis factor ligand superfamily member 5, CD antigen CD154 Relevance:Mediates B-cell proliferation in the absence of co-stimµLus as well as IgE production in the presence of IL-4. Involved in immunoglobµLin class switching. {ECO:0000269|PubMed:15193700}.; Release of soluble CD40L from platelets is partially regµLated by GP IIb/IIIa, actin polymerization, and an matrix metalloproteinases (MMP) inhibitor-sensitive pathway. {ECO:0000269|PubMed:15193700}. PubMed ID:1280226; 1385114; 7678782; 1281209; 7678552; 7999797; 15489334; 8626375; 11676606; 15193700; 8589998; 9605317; 7679206; 8094231; 7679801; 7717401; 7532185; 8889581; 8550833; 9150729; 9746782 Function:Cytokine that binds to CD40/TNFRSF5 Involvement in disease:Immunodeficiency with hyper-IgM, type 1 (HIGM1) SubcellµLar Location:Cell membrane, Single-pass type II membrane protein, Cell surface, SUBCELLµLAR LOCATION: CD40 ligand, soluble form: Secreted Protein Families:Tumor necrosis factor family Tissue Specificity:Specifically expressed on activated CD4+ T-lymphocytes. Paythway:NF-kappaBsignalingpathway HGNC Database Link:https://www.genenames.org/cgi-bin/gene_symbol_report?hgnc_id=HGNC:11935 UniGene Database Link:https://www.ncbi.nlm.nih.gov/UniGene/clust.cgi?ORG=Hs&CID=592244 KEGG Database Link:https://www.genome.jp/dbget-bin/www_bget?hsa:959 STRING Database Link:https://string-db.org/network/9606.ENSP00000359663 OMIM Database Link:https://www.omim.org/entry/300386300386300386