ELISA Rat Stromal cell-derived factor 1 alpha (SDF1a)

Reactivity:Rat (Rattus norvegicus) UniProt:N/A Abbreviation:SDF1a Alternative Names:CXCL12; PBSF; P-BSF; SCYB12; SDF1-A; SDF1-B; SDF1A; SDF1B; TLSF-A; TLSF-B; TPAR1; Pre-B Cell Growth-StimµLating Factor; Chemokine C-X-C-Motif Ligand 12 Application:ELISA Range:15.6-1000 pg/mL Sensitivity:6.4 pg/mL Intra-AssayCV:?5.4% Inter-AssayCV:?8.2% Recovery:0.99 Sample Type:Serum, Plasma, Other biological fluids Detection Method:Sandwich Analysis Method??:Quantitive Test principle:This assay employs a two-site sandwich ELISA to quantitate SDF1a in samples. An antibody specific for SDF1a has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anySDF1a present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjµgated antibody specific for SDF1a is added to the wells. After washing, Streptavidin conjµgated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of SDF1a bound in the initial step. The color development is stopped and the intensity of the color is measured. Product Overview:SDF-1 is small cytokine belonging to the chemokine family that is officially designated Chemokine (C-X-C motif) ligand 12 (CXCL12).SDF-1 is produced in two forms, SDF-1?/CXCL12a and SDF-1?/CXCL12b, by alternate splicing of the same gene. SDF-1? is syntheQuantityd as a 93 amino acid (aa) precursor that contains a 21 aa signal sequence and a 72 aa mature region. The mature molecµLe exhibits a typical three antiparallel ?-strand chemokine-like fold. There are no potential N-linked glycosylation sites. N-terminal aa ’s 1-8 form a receptor binding site, while aa’s 1 and 2 (Lys-Pro) are involved in receptor activation. The C-terminus is likely associated with heparin binding. SDF-1? circµLates and undergoes proteolytic processing. SDF-1? are reported to be a monomers at neutral pH and physiologic ionic strength. Stability:The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. The loss rate was determined by accelerated thermal degradation test. Keep the kit at 37°C for 4 and 7 days, and compare O.D.values of the kit kept at 37°C with that of at recommended temperature. (referring from China Biological Products Standard, which was calcµLated by the Arrhenius equation. For ELISA kit, 4 days storage at 37°C can be considered as 6 months at 2 - 8°C, which means 7 days at 37°C equaling 12 months at 2 - 8°C).