ELISA Mouse E-Selectin (SELE)

Reactivity:Mouse (Mus muscµLus) UniProt:Q00690 Abbreviation:SELE Alternative Names:RP1-117P20.2; CD62E; ELAM; ELAM1; ESEL; LECAM2; endothelial adhesion molecµLe 1|leukocyte endothelial cell adhesion molecµLe 2 Application:ELISA Range:0.156-10 ng/mL Sensitivity:0.065 ng/mL Intra-AssayCV:?5.1% Inter-AssayCV:?7.1% Recovery:1.03 Sample Type:Serum, Plasma, Other biological fluids Detection Method:Sandwich Analysis Method??:Quantitive Test principle:This assay employs a two-site sandwich ELISA to quantitate SELE in samples. An antibody specific for SELE has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anySELE present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjµgated antibody specific for SELE is added to the wells. After washing, Streptavidin conjµgated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of SELE bound in the initial step. The color development is stopped and the intensity of the color is measured. Product Overview:CD62E is also known as endothelial leukocyte adhesion molecµLe 1 (ELAM1) or E selectin. It is a type I membrane protein that belongs to the selectin family of surface molecµLes (along with CD62L and CD62P). Selectins are C type cell surface lectins that play a role in leukocyte adhesion to the blood vessel wall endothelium. CD62E (E selectin) is an endothelial cell specific selectin that is expressed on cytokine induced endothelial cells only after activation with proinflammatory cytokines. In vitro experiments have shown that IL1, TNFalpha and bacterial wall components like lipopolysaccharides induce the transcription of CD62E in an NFkB dependent signalling cascade. CD62E has been associated with blood vessel endothelium in diverse inflammatory situations. Stability:The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. The loss rate was determined by accelerated thermal degradation test. Keep the kit at 37°C for 4 and 7 days, and compare O.D.values of the kit kept at 37°C with that of at recommended temperature. (referring from China Biological Products Standard, which was calcµLated by the Arrhenius equation. For ELISA kit, 4 days storage at 37°C can be considered as 6 months at 2 - 8°C, which means 7 days at 37°C equaling 12 months at 2 - 8°C).