ELISA Rat Slit homolog 2 protein (SLIT2)

Reactivity:Rat (Rattus norvegicus) UniProt:Q9WVC1 Abbreviation:SLIT2 Alternative Names:FLJ14420; SLIL3; Slit-2; slit homolog 2 Application:ELISA Range:78.12-5000 pg/mL Sensitivity:30 pg/mL Intra-AssayCV:?6.2% Inter-AssayCV:?9.8% Recovery:1 Sample Type:Serum, Plasma, Other biological fluids Detection Method:Sandwich Analysis Method??:Quantitive Test principle:This assay employs a two-site sandwich ELISA to quantitate SLIT2 in samples. An antibody specific for SLIT2 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anySLIT2 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjµgated antibody specific for SLIT2 is added to the wells. After washing, Streptavidin conjµgated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of SLIT2 bound in the initial step. The color development is stopped and the intensity of the color is measured. Product Overview:Slit2 plays a vital role in axon guidance by signaling throµgh Robo receptors. Recent evidence sµggests that Slit2 protein may function in other settings because and Xenopus Slit2 has been shown to inhibit leukocyte chemotaxis. SLIT2 protein is a putative ligand for the ROBO receptors. ROBO1 is inactivated by promoter region hypermethylation in <20% of cancers. Slit2 is an excellent candidate tumor suppressor gene for colorectal cancer?and that it epigenetically silenced in >40% of lung and breast tumors. SLIT2 promoter region methylation was found in 23 (72%) of 32 primary colorectal cancers. In contrast, normal colorectal mucosa from the same patients exhibited significantly lower levels of SLIT2 promoter region hypermethylation. Stability:The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. The loss rate was determined by accelerated thermal degradation test. Keep the kit at 37°C for 4 and 7 days, and compare O.D.values of the kit kept at 37°C with that of at recommended temperature. (referring from China Biological Products Standard, which was calcµLated by the Arrhenius equation. For ELISA kit, 4 days storage at 37°C can be considered as 6 months at 2 - 8°C, which means 7 days at 37°C equaling 12 months at 2 - 8°C).